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Addgene

Bcr GAP domain/pGEX 3X
(Plasmid #36405)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36405 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX 3X
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bcr encoding residues 1004-1271
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sma I (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We subcloned a Pvu II-EcoRI fragment into pGEX-3X digested with Sma I -EcoRI. This includes the natural stop codon and 3' untranslated sequences. The construct encodes the GTPase activating domain of Bcr which is active against GTP-bound Rac. The Pvu II site is located in the amino acid string -QLDP-. Constructed by Vesa Kaartinen. The Rosetta2 strain may improve yield of the fusion protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Bcr GAP domain/pGEX 3X was a gift from Nora Heisterkamp (Addgene plasmid # 36405 ; http://n2t.net/addgene:36405 ; RRID:Addgene_36405)
  • For your References section:

    Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N. Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20. 10.1128/MCB.00756-06 PubMed 17116687