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pGEX-2T Crkl SH2SH3
(Plasmid #36404)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2T
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Crkl SH2 and N-terminal SH3 domains
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    550
  • Entrez Gene
    CRKL
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site TaqI into SmaI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct includes the SH2 domain and N- terminal SH3 domains of Crkl, including residues 7-195. Constructed by Johanna ten Hoeve. Reference: PMID 9053848.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2T Crkl SH2SH3 was a gift from Nora Heisterkamp (Addgene plasmid # 36404 ; http://n2t.net/addgene:36404 ; RRID:Addgene_36404)

  • For your References section:

    Crkl is complexed with tyrosine-phosphorylated Cbl in Ph-positive leukemia. de Jong R, ten Hoeve J, Heisterkamp N, Groffen J. J Biol Chem. 1995 Sep 15;270(37):21468-71. 10.1074/jbc.270.37.21468 PubMed 7545163
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