pGEX-2T Crkl
(Plasmid
#36400)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2T
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCrkl
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SpeciesH. sapiens (human)
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Entrez GeneCRKL
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX-5
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Encodes amino acid residues 7-303. Insert starts at a 5' Taq I site in the human cDNA. Constructed by Johanna ten Hoeve. Refrerences: PMID 8168080, 7545163 and 9053848.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-2T Crkl was a gift from Nora Heisterkamp (Addgene plasmid # 36400 ; http://n2t.net/addgene:36400 ; RRID:Addgene_36400) -
For your References section:
Cellular interactions of CRKL, and SH2-SH3 adaptor protein. ten Hoeve J, Kaartinen V, Fioretos T, Haataja L, Voncken JW, Heisterkamp N, Groffen J. Cancer Res. 1994 May 15;54(10):2563-7. PubMed 8168080