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Addgene

alpha-0 CFP in pcDNA3.1
(Plasmid #36396)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36396 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7291
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G protein alpha 0
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1065
  • Mutation
    CFP inserted into the KpnI-SpeI sites engineered between a.a. 92 and 93 of alpha 0
  • GenBank ID
    NM_017327.1
  • Entrez Gene
    Gnao1 (a.k.a. Gnao, Hg1g, RATBPGTPC)
  • Promoter cmv
  • Tag / Fusion Protein
    • CFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    alpha-0 CFP in pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 36396 ; http://n2t.net/addgene:36396 ; RRID:Addgene_36396)

  • For your References section:

    A fluorescence resonance energy transfer-based sensor indicates that receptor access to a G protein is unrestricted in a living mammalian cell. Azpiazu I, Gautam N. J Biol Chem. 2004 Jun 25;279(26):27709-18. Epub 2004 Apr 12. 10.1074/jbc.M403712200 PubMed 15078878
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