pUX19
(Plasmid
#36328)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36328 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUK21
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Backbone manufacturerVieira and Messing, 1991
- Backbone size (bp) 3868
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Modifications to backboneA 778bp oriT (Mob) HaeII fragment from pSUP202 was cloned into pUK21 at an HaeII site upstream of the lac region.
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Vector typeBacterial Expression ; Suicide vector for R. rubrum
- Promoter reduce strength lacZ
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer none
- 3′ sequencing primer LacI-R or LacZ-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructed by D. P. Lies and G. P. Roberts
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUX19 was a gift from Gary Roberts (Addgene plasmid # 36328 ; http://n2t.net/addgene:36328 ; RRID:Addgene_36328)