pcDNA-Golgi-ZapCY1
(Plasmid #36322)

• Depositing Lab: Amy Palmer
• Publication: Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Epub 2011 Apr 18.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Golgi-ZapCY1 high affinity Zn(II) sensor
• Species: Synthetic
• Insert Size (bp): 1836
• GenBank ID: JF261179.1
• Promoter: CMV
• Tag / Fusion Protein:
  • GalT (first 60 amino acids) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: BGH Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.

Targeted to Golgi: N-term fusion of the first 60 amino acids of GalT