pcDNA-ZifCY3 (Ala4)
(Plasmid #36318)

• Depositing Lab: Amy Palmer
• Publication: Dittmer et al J Biol Chem. 2009 Jun 12;284(24):16289-97. Epub 2009 Apr 10.

Backbone

• Vector backbone: pcDNA3'
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZifCY3 genetically encoded Zn(II) sensor
• Alt name: Ala4
• Species: Synthetic
• Insert Size (bp): 1515
• Mutation: see comment
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: BGH Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. The two cysteines and two histidines that coordinate Zn(II) have been mutated to alanines. This sensor does not bind Zn(II).