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Addgene

alpha 0-CFP in pdest12.2
(Plasmid #36312)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36312 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pd12.2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7261
  • Total vector size (bp) 9052
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G protein alpha 0
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1065
  • Mutation
    Introduced KpnI and SpeI sites between 92nd and 93rd amino acids of alpha 0
  • GenBank ID
    NM_017327.1
  • Entrez Gene
    Gnao1 (a.k.a. Gnao, Hg1g, RATBPGTPC)
  • Promoter CMV
  • Tag / Fusion Protein
    • CFP

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The CFP was introduced between 92nd and 93rd amino acid of alpha-0

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    alpha 0-CFP in pdest12.2 was a gift from Narasimhan Gautam (Addgene plasmid # 36312 ; http://n2t.net/addgene:36312 ; RRID:Addgene_36312)

  • For your References section:

    A fluorescence resonance energy transfer-based sensor indicates that receptor access to a G protein is unrestricted in a living mammalian cell. Azpiazu I, Gautam N. J Biol Chem. 2004 Jun 25;279(26):27709-18. Epub 2004 Apr 12. 10.1074/jbc.M403712200 PubMed 15078878
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