USP19 (C506A)-FLAG/pRK5
(Plasmid
#36307)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36307 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameubiquitin specific peptidase 19
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Alt nameUSP19
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Alt nameKIAA0891
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SpeciesH. sapiens (human)
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MutationC506A
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Entrez GeneUSP19 (a.k.a. ZMYND9)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
USP19 (C506A)-FLAG/pRK5 was a gift from Xiaolu Yang (Addgene plasmid # 36307 ; http://n2t.net/addgene:36307 ; RRID:Addgene_36307) -
For your References section:
The USP19 deubiquitinase regulates the stability of c-IAP1 and c-IAP2. Mei Y, Hahn AA, Hu S, Yang X. J Biol Chem. 2011 Oct 14;286(41):35380-7. Epub 2011 Aug 17. 10.1074/jbc.M111.282020 PubMed 21849505