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Addgene

USP19-Flag/pRK5
(Plasmid #36306)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36306 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ubiquitin specific peptidase 19
  • Alt name
    USP19
  • Alt name
    KIAA0891
  • Species
    H. sapiens (human)
  • Entrez Gene
    USP19 (a.k.a. ZMYND9)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    USP19-Flag/pRK5 was a gift from Xiaolu Yang (Addgene plasmid # 36306 ; http://n2t.net/addgene:36306 ; RRID:Addgene_36306)

  • For your References section:

    The USP19 deubiquitinase regulates the stability of c-IAP1 and c-IAP2. Mei Y, Hahn AA, Hu S, Yang X. J Biol Chem. 2011 Oct 14;286(41):35380-7. Epub 2011 Aug 17. 10.1074/jbc.M111.282020 PubMed 21849505
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