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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36267 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD33
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Modifications to backboneReplaced pBAD33 nucleotides between XbaI to HindIII with those of pET21b including ribosomal binding site
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Vector typeBacterial Expression
- Promoter PBAD
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Cloning Information
- 5′ sequencing primer pBAD-F (5'-ATGCCATAGCATTTTTATCC-3')
- 3′ sequencing primer pBAD-R (5'-GATTTAATCTGTATCAGG-3') (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD33.1 was a gift from Christian Raetz (Addgene plasmid # 36267 ; http://n2t.net/addgene:36267 ; RRID:Addgene_36267) -
For your References section:
Interchangeable domains in the Kdo transferases of Escherichia coli and Haemophilus influenzae. Chung HS, Raetz CR. Biochemistry. 2010 May 18;49(19):4126-37. 10.1021/bi100343e PubMed 20394418
