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HA-mDyn2 pcDNA3
(Plasmid #36264)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36264 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dynamin 2
  • Alt name
    Dyn2
  • Alt name
    mDyn2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Dnm2 (a.k.a. Dyn2, Udnm, b2b2159Clo)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HA-tagged mouse dynamin 2 flanked by KpnI and XbaI sites. BamHI between N-terminal HA tag and beginning of dynamin coding seq.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-mDyn2 pcDNA3 was a gift from Pietro De Camilli (Addgene plasmid # 36264 ; http://n2t.net/addgene:36264 ; RRID:Addgene_36264)

  • For your References section:

    A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Ferguson SM, Brasnjo G, Hayashi M, Wolfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, Flavell R, Cremona O, Miesenbock G, Ryan TA, De Camilli P. Science. 2007 Apr 27;316(5824):570-4. 10.1126/science.1140621 PubMed 17463283
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