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Addgene

pCMV_eYFP-eCFP
(Plasmid #36262)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36262 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    peYFP
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 5423
  • Modifications to backbone
    eCFP was added in fusion with eYFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eYFP-eCFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    1476
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV_eYFP-eCFP was a gift from Isabelle Richard (Addgene plasmid # 36262)

  • For your References section:

    Imaging calpain protease activity by multiphoton FRET in living mice. Stockholm D, Bartoli M, Sillon G, Bourg N, Davoust J, Richard I. J Mol Biol. 2005 Feb 11;346(1):215-22. Epub 2004 Dec 16. 10.1016/j.jmb.2004.11.039 PubMed 15663939
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