pRiboS EcoHind
(Plasmid
#36254)
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Purpose(Empty Backbone)
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36254 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMV261
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Backbone manufacturerStover et al., 1991 Nature 351:456
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Modifications to backboneThe plasmid is derived from pMV261 (http://www.lablife.org/p?a=vdb_view&id=g2.7281169b992e40c865698d3455e3633c24b04b31). It contains a modified promoter.
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Vector typeBacterial Expression, Synthetic Biology
- Promoter Modified Hsp60
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 3′ sequencing primer rrnB-T1-term-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid lacks the pAL5000 origin of replication and does not replicate in mycobacteria. This plasmid is related to pRiboS BsaHind, but is not reported in PMID 2227955333: Unlike pRiboS BsaHind, the original MCS is preserved, except that the BstBI site is lost.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRiboS EcoHind was a gift from Carolyn Bertozzi & Jessica Seeliger (Addgene plasmid # 36254 ; http://n2t.net/addgene:36254 ; RRID:Addgene_36254) -
For your References section:
A riboswitch-based inducible gene expression system for mycobacteria. Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18. 10.1371/journal.pone.0029266 PubMed 22279533
