ITCH
(Plasmid #36197)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q3&Q4 2011 (unpublished)

Backbone

• Vector backbone: pET28-MHL (Addgene plasmid 26096)
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7325
• Total vector size (bp): 8465
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ITCH
• Alt name: PDB: 3TUG
• Alt name: itchy E3 ubiquitin protein ligase homolog, HECT domain
• Alt name: AIF4, AIP4, dJ468O1.1, NAPP1
• Species: H. sapiens (human)
• Insert Size (bp): 1140
• Mutation: contains HECT domain
• GenBank ID: BC011571
• Entrez Gene: ITCH (a.k.a. ADMFD, AIF4, AIP4, NAPP1)
• Tag / Fusion Protein:
  • His tag with TEV cleavage (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3TUG. http://www.thesgc.org/structures/3TUG/