AMPKA2
(Plasmid #36194)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q3&Q4 2011 (unpublished)

Backbone

• Vector backbone: pET28a-LIC (Addgene plasmid 26094)
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Total vector size (bp): 7583
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: AMPKA2
• Alt name: PDB: 2H6D
• Alt name: Kinase Domain of Human AMP-activated Protein Kinase alpha2
• Alt name: AMPK, AMPK2, PRKAA
• Species: H. sapiens (human)
• Insert Size (bp): 255
• Mutation: contains KINASE DOMAIN only
• GenBank ID: NP_006243, BC069823
• Entrez Gene: PRKAA2 (a.k.a. AMPK, AMPK2, AMPKa2, PRKAA)
• Tag / Fusion Protein:
  • His tag with thrombin cleavage (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 2h6d. http://www.thesgc.org/structures/2H6D/