pEGFP.N1-HDAC6.delta Buz
(Plasmid #36190)

• Depositing Lab: Tso-Pang Yao
• Publication: Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-26.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HDAC6..delta Buz
• Species: H. sapiens (human)
• Mutation: Deletion of the C-terminal ub binding domain (BUZ finger): aa1044-1215
• Entrez Gene: HDAC6 (a.k.a. CPBHM, HD6, JM21, KDAC6, PPP1R90)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry