pLVX-Puro-mRuby-Sec23A
(Plasmid #36158)

• Depositing Lab: David Stephens
• Publication: Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8. Epub 2010 Nov 2.

Backbone

• Vector backbone: pLVX-Puro
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sec23A
• Species: H. sapiens (human)
• Entrez Gene: SEC23A (a.k.a. CLSD, hSec23A)
• Promoter: CMV
• Tag / Fusion Protein:
  • mRuby (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (destroyed during cloning)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: CMV
• 3′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • Seqbuilder file of the sequence
• A portion of this plasmid was derived from a plasmid made by: mRuby was synthesized to our requirements by Eurofins MWG
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The cDNA sequence encoding mRuby (Kredel et al., 2009) underwent codon usage
adaptation before gene synthesis (MWG Eurofins) and cloning into pBluescript II
SK(+). mRuby was excised with SalI-EcoRI for insertion into pLVX-Puro (Clontech)
to generate pLVX-Puro–mRuby. A cDNA encoding human Sec23A was amplified
by PCR, gel purified and subcloned via pGEM-T vector (excised with ApaI-XbaI)
and cloned into pLVX-Puro–mRuby.