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Addgene

pVenus-Sec16A
(Plasmid #36157)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36157 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVenus-C1
  • Backbone manufacturer
    Clontech, then modified
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec16A
  • Alt name
    KIAA0310
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEC16A (a.k.a. KIAA0310, SEC16L, p250)
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus (EYFP derivative) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original insert from Kasuza as detailed in the attachment. Cloned by Peter Watson, now at University of Cardiff when he was a postdoc in the lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVenus-Sec16A was a gift from David Stephens (Addgene plasmid # 36157 ; http://n2t.net/addgene:36157 ; RRID:Addgene_36157)

  • For your References section:

    Sec16 defines endoplasmic reticulum exit sites and is required for secretory cargo export in mammalian cells. Watson P, Townley AK, Koka P, Palmer KJ, Stephens DJ. Traffic. 2006 Dec;7(12):1678-87. Epub 2006 Sep 27. 10.1111/j.1600-0854.2006.00493.x PubMed 17005010
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