unc5bCD4d3+4-blac
(Plasmid #36149)

• Purpose: EXPRESs plasmid for Zebrafish Receptors encoding zebrafish unc5b (netrin-1a) with rat CD4d3+4-blac tag
• Depositing Lab: Gavin Wright
• Publication: Sollner et al Genome Biol. 2009;10(9):R99. Epub 2009 Sep 18.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 9000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: unc5b
• Alt name: unc5b
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1150
• Mutation: L2V
• Entrez Gene: unc5b (a.k.a. unc5h2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • ratCD4d3+4 (C terminal on backbone)
  • pentamerization domain of rat cartilaginous oligomeric matrix protein (COMP) (C terminal on backbone)
  • beta-lactamase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV Foward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains an intentional L2V mutation in the unc5b sequence to create a consensus Kozak sequence.