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Addgene

PcDNA-DEST47- GFP-GFP
(Plasmid #36139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36139 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA-DEST47
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7780
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct contains EGFP-GFP (EGFP was inserted into the pcDNA-DEST47 vector backbone which already contains a C-terminal GFP). This construct was used as a control in transection experiments.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PcDNA-DEST47- GFP-GFP was a gift from Patrick Van Oostveldt (Addgene plasmid # 36139)

  • For your References section:

    Spatiotemporal behavior of nuclear cyclophilin B indicates a role in RNA transcription. Dieriks B, Van Oostveldt P. Int J Mol Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937. 10.3892/ijmm.2012.937 PubMed 22426501
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