PcDNA-DEST47-CypB-Del3 C-term GFP
(Plasmid #36136)

• Depositing Lab: Patrick Van Oostveldt
• Publication: Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937.

Backbone

• Vector backbone: pcDNA-DEST47
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7780
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyclophilin B
• Alt name: Peptidyl-prolyl cis-trans isomerase B
• Species: H. sapiens (human)
• Mutation: contains amino acids 39-216 of cyclophilin B; G63S, M176T and F211L relative to NP_000933.1
• Entrez Gene: PPIB (a.k.a. CYP-S1, CYPB, HEL-S-39, OI9, SCYLP)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified G63S, M176T and F211L mutations when compared to GenBank reference sequence NP_000933.1