PcDNA-DEST47-CypB-Iso C-term GFP
(Plasmid
#36130)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36130 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA-DEST47
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7780
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecyclophilin B
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Alt namePeptidyl-prolyl cis-trans isomerase B
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SpeciesH. sapiens (human)
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Mutationcontains amino acids 47-204 of cyclophilin B
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Entrez GenePPIB (a.k.a. CYP-S1, CYPB, HEL-S-39, OI9, SCYLP)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PcDNA-DEST47-CypB-Iso C-term GFP was a gift from Patrick Van Oostveldt (Addgene plasmid # 36130 ; http://n2t.net/addgene:36130 ; RRID:Addgene_36130) -
For your References section:
Spatiotemporal behavior of nuclear cyclophilin B indicates a role in RNA transcription. Dieriks B, Van Oostveldt P. Int J Mol Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937. 10.3892/ijmm.2012.937 PubMed 22426501