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Addgene

mcherry Beta 4
(Plasmid #36114)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36114 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7162
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G protein beta subunit B4
  • Alt name
    GNB4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1023
  • Entrez Gene
    Gnb4 (a.k.a. 6720453A21Rik, G(beta)4)
  • Promoter cmv
  • Tag / Fusion Protein
    • mcherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH reverse primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mcherry Beta 4 was a gift from Narasimhan Gautam (Addgene plasmid # 36114 ; http://n2t.net/addgene:36114 ; RRID:Addgene_36114)

  • For your References section:

    A family of G protein betagamma subunits translocate reversibly from the plasma membrane to endomembranes on receptor activation. Saini DK, Kalyanaraman V, Chisari M, Gautam N. J Biol Chem. 2007 Aug 17;282(33):24099-108. Epub 2007 Jun 20. 10.1074/jbc.M701191200 PubMed 17581822
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