pEBB-SBP-HA
(Plasmid
#36100)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 36100 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEBB
- Backbone size (bp) 5340
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Vector typeMammalian Expression
- Promoter EF1a
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Tags
/ Fusion Proteins
- SBP (N terminal on backbone)
- HA (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer ctcaagcctcagacagtgg
- 3′ sequencing primer GAGGGGTGCAAGGACCCAG (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SBP - Streptavidin binding peptide tag, biotinilated in vivo
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEBB-SBP-HA was a gift from Ezra Burstein (Addgene plasmid # 36100 ; http://n2t.net/addgene:36100 ; RRID:Addgene_36100)