pEBB-TB
(Plasmid #36099)

• Purpose: (Empty Backbone)
• Depositing Lab: Ezra Burstein
• Publication: Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22.

Backbone

• Vector backbone: pEBB
• Backbone size (bp): 5450
• Modifications to backbone: BT-tag (biotinilation site from P.freudenreichia transcarboxilase, separated from MCS by TEV-site)
• Vector type: Mammalian Expression
• Promoter: EF1a
• Tag / Fusion Protein:
  • BT-tag (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CTCAAGCCTCAGACAGTGG
• 3′ sequencing primer: GAGGGGTGCAAGGACCCAG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

After mammal transfection, supplement fresh media with 4 uM Biotin to "charge" tag by Biotin. Allow cells to grow for about 48 h before the purification steps.