pEBB-BT-Ubiquitin
(Plasmid #36098)

• Depositing Lab: Ezra Burstein
• Publication: Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22.

Backbone

• Vector backbone: pEBB-TB
• Backbone size w/o insert (bp): 5416
• Total vector size (bp): 5894
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin
• Alt name: Ubi
• Alt name: RPS27A
• Alt name: UBC
• Species: H. sapiens (human)
• Insert Size (bp): 228
• Entrez Gene: RPS27A (a.k.a. CEP80, HEL112, S27A, UBA80, UBC, UBCEP1, UBCEP80, eS31)
• Promoter: EF1a
• Tag / Fusion Protein:
  • TB-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: ctcaagcctcagacagtgg
• 3′ sequencing primer: GAGGGGTGCAAGGACCCAG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

After transfection into mammal cells, supplement fresh media with 4 uM Biotin and allow cells to grow for about 48 h before the purification procedures.