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Purpose(Empty Backbone) 3rd generation lentiviral plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36097 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRRLsinPPT-CMV-MCS-WPRE
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Backbone manufacturerNaldini Lab
- Backbone size (bp) 6782
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Modifications to backbonenew polylinker (MP2)
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsoptimal growth in low salt LB (5gm NaCl)
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Copy numberUnknown
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-MP2 was a gift from Pantelis Tsoulfas (Addgene plasmid # 36097 ; http://n2t.net/addgene:36097 ; RRID:Addgene_36097) -
For your References section:
A multifunctional neurotrophin with reduced affinity to p75NTR enhances transplanted Schwann cell survival and axon growth after spinal cord injury. Enomoto M, Bunge MB, Tsoulfas P. Exp Neurol. 2013 Oct;248:170-82. doi: 10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20. 10.1016/j.expneurol.2013.06.013 PubMed 23792206