pRS424GFP-FYVE(EEA1)
(Plasmid #36096)

• Depositing Lab: Scott Emr
• Publication: Burd et al Mol Cell. 1998 Jul;2(1):157-62.

Backbone

• Vector backbone: pRS424
• Modifications to backbone: To create a yeast GFP expression vector (pGOGFP), the PRC1 promoter and GFP coding sequences were cloned into the BamHI site of pRS424.
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FYVE(EEA1) domain
• Species: H. sapiens (human)
• Entrez Gene: EEA1 (a.k.a. MST105, MSTP105, ZFYVE2)
• Promoter: PRC1
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unkown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry