pLV-Azurite
(Plasmid #36086)

• Purpose: (Empty Backbone) 3rd generation lentiviral plasmid
• Depositing Lab: Pantelis Tsoulfas
• Publication: Tsoulfas Lab plasmids (unpublished)

Backbone

• Vector backbone: pLenti-MP2
• Backbone size (bp): 6782
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: optimal growth in low salt LB (5gm NaCl)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Azurite
• Alt name: enhanced blue fluorescent protein, BFP, eB
• Species: Synthetic
• Insert Size (bp): 734

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The Azurite was obtained from Addgene and is originally from Patrick Daugherty (Mena et al., Nat Biotech 2006).
• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.

Azurite is mammalian codon optimized.