pLV-EBFP2-nuc
(Plasmid #36085)

• Purpose: (Empty Backbone)
• Depositing Lab: Pantelis Tsoulfas
• Publication: Tsoulfas Lab plasmids (unpublished)

Backbone

• Vector backbone: pLenti-MP2
• Backbone size (bp): 6782
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: optimal growth in low salt LB (5gm NaCl)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pEBFP2-Nuc
• Alt name: EBFP2 blue fluorescent protein with NLS
• Species: Synthetic; evolved fluorescent protein
• Insert Size (bp): 720
• Mutation: Replace EYFP of the vector pEYFP-Nuc from Clontech with EBFP2.
• GenBank ID: EF517318
• Tag / Fusion Protein:
  • NLS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The EBFP2-nuc was obtained from Addgene and was originally from Robert Campbell's group (Ai HW et al. Biochemistry 2007).
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.