YFP-Gamma 9-3
(Plasmid #36074)

• Depositing Lab: Narasimhan Gautam
• Publication: Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6401
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G protein G9(gamma 3 tail)
• Alt name: Gng9-3
• Species: B. taurus (bovine)
• Insert Size (bp): 219
• Mutation: HIs tag between YFP and insert
• GenBank ID: NM_174328.2
• Promoter: CMV
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: BGH reverse primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mutagenesis was used to replace the last 15 residues of gamma 9 with those of gamma 3.