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Addgene

aq-CFP
(Plasmid #36073)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36073 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G protein subunit alpha q
  • Alt name
    Gnaq
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1077
  • Mutation
    CFP inserted between amino acid 124 and 125
  • GenBank ID
    NM_008139
  • Entrez Gene
    Gnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, Dsk1, Dsk10, Galphaq, Gq, GqI)
  • Promoter CMV
  • Tag / Fusion Protein
    • CFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    aq-CFP was a gift from Narasimhan Gautam (Addgene plasmid # 36073 ; http://n2t.net/addgene:36073 ; RRID:Addgene_36073)

  • For your References section:

    G protein subunit dissociation and translocation regulate cellular response to receptor stimulation. Chisari M, Saini DK, Cho JH, Kalyanaraman V, Gautam N. PLoS One. 2009 Nov 11;4(11):e7797. 10.1371/journal.pone.0007797 PubMed 19936219
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