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Addgene

YFP-Gamma 5
(Plasmid #36044)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G protein subunit Gamma 5
  • Alt name
    Gng5
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    207
  • GenBank ID
    NM_024377.1
  • Entrez Gene
    Gng5
  • Promoter CMV
  • Tags / Fusion Proteins
    • YFP (N terminal on backbone)
    • His

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH reverse primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP-Gamma 5 was a gift from Narasimhan Gautam (Addgene plasmid # 36044 ; http://n2t.net/addgene:36044 ; RRID:Addgene_36044)

  • For your References section:

    G protein betagamma complex translocation from plasma membrane to Golgi complex is influenced by receptor gamma subunit interaction. Akgoz M, Kalyanaraman V, Gautam N. Cell Signal. 2006 Oct;18(10):1758-68. Epub 2006 Mar 6. 10.1016/j.cellsig.2006.01.016 PubMed 16517125
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