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Addgene

pTAL6-BB
(Plasmid #36034)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36034 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTAL5-BB
  • Backbone size (bp) 6881
  • Modifications to backbone
    Removed PGAL1 and replaced with PTEF1 cloned in as an AvrII/EcoRI fragment
  • Vector type
    Yeast Expression
  • Promoter PTEF1
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid derived from pTAL3 from Addgene TALEN Golden Gate cloning kit.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information on Ellis TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#ellis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAL6-BB was a gift from Tom Ellis (Addgene plasmid # 36034 ; http://n2t.net/addgene:36034 ; RRID:Addgene_36034)

  • For your References section:

    Rational diversification of a promoter providing fine-tuned expression and orthogonal regulation for synthetic biology. Blount BA, Weenink T, Vasylechko S, Ellis T. PLoS One. 2012;7(3):e33279. Epub 2012 Mar 19. 10.1371/journal.pone.0033279 PubMed 22442681
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