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Addgene

pTAL5-BB
(Plasmid #36033)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36033 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTAL3
  • Backbone manufacturer
    Addgene
  • Backbone size (bp) 7530
  • Modifications to backbone
    The pTAL5 plasmid was constructed using the Gibson method to assemble four sections into an ordered plasmid; 1 - PGAL1 was amplified from S. cerevisiae YPH500 genomic DNA. 2 - The TALEN destination protein coding sequence minus the FokI domain was amplified from pTAL3. 3 - The ADH1 terminator was amplified from pTVGI. 4 - The pTAL3 vector backbone was amplified as a linear fragment.
  • Vector type
    Yeast Expression
  • Promoter PGAL1
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid derived from pTAL3 from Addgene TALEN Golden Gate cloning kit.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information on Ellis TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#ellis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAL5-BB was a gift from Tom Ellis (Addgene plasmid # 36033 ; http://n2t.net/addgene:36033 ; RRID:Addgene_36033)

  • For your References section:

    Rational diversification of a promoter providing fine-tuned expression and orthogonal regulation for synthetic biology. Blount BA, Weenink T, Vasylechko S, Ellis T. PLoS One. 2012;7(3):e33279. Epub 2012 Mar 19. 10.1371/journal.pone.0033279 PubMed 22442681
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