Protein Trap
(Plasmid
#36009)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36009 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFLAG-CMV2
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameΔC1LAP2
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MutationΔC1LAP2 is residues 1-398 of LAP2β
- Promoter CMV
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Tags
/ Fusion Proteins
- FKBP12 (N terminal on insert)
- FKBP12 (N terminal on insert)
- FKBP12 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To be used in conjunction with the reporter construct (Addgene plasmid #36008).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Protein Trap was a gift from Larry Gerace (Addgene plasmid # 36009 ; http://n2t.net/addgene:36009 ; RRID:Addgene_36009) -
For your References section:
Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore. Ohba T, Schirmer EC, Nishimoto T, Gerace L. J Cell Biol. 2004 Dec 20;167(6):1051-62. 10.1083/jcb.200409149 PubMed 15611332