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Addgene

Reporter
(Plasmid #36008)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36008 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression ; Reporter
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LAP2β-TM
  • Mutation
    FRB is residues 2025-2114 of FRAP, LAP2β-TM is residues 401-452 of LAP2β
  • Promoter CMV
  • Tags / Fusion Proteins
    • 11kD FRB (N terminal on insert)
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV fwd
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To be used in conjunction with protein trap construct (Addgene plasmid #36009).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Reporter was a gift from Larry Gerace (Addgene plasmid # 36008 ; http://n2t.net/addgene:36008 ; RRID:Addgene_36008)

  • For your References section:

    Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore. Ohba T, Schirmer EC, Nishimoto T, Gerace L. J Cell Biol. 2004 Dec 20;167(6):1051-62. 10.1083/jcb.200409149 PubMed 15611332
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