Park2_L (TAL 3012)
(Plasmid #36004)

• Depositing Lab: Keith Joung
• Publication: Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.

Backbone

• Vector backbone: JDS 74
• Vector type: Mammalian Expression ; T7

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TAL array targeting Park2
• Alt name: Park2
• Species: D. rerio (zebrafish)
• Entrez Gene: prkn (a.k.a. p, pa, park2, si:ch211-123f21.1, zgc:112390)
• Tags / Fusion Proteins:
  • 3X Flag (N terminal on backbone)
  • WT FOKI (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: CMV-f
• 3′ sequencing primer: BGH rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results identified a single nucleotide mismatch at bp# 3873 when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing laboratory, this mismatch does not affect the function of the plasmid.