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Addgene

pUAS-Ncad-GFP
(Plasmid #35986)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35986 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAS-EGFP
  • Backbone manufacturer
    Dev Biol. 2001 May 15;233(2):329-46
  • Modifications to backbone
    The backbone is essentially pEGFP-N1, where the CMV was deleted and the UAS cassette was inserted between HindIII-EcoR1
  • Vector type
    Gal4 inducible
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ncad
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    cdh2 (a.k.a. bib, glo, lyr, ncad, pac, wu:fb47h04)
  • Promoter 14xUAS-E1b
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAS-Ncad-GFP was a gift from James Jontes & Stephen Smith (Addgene plasmid # 35986 ; http://n2t.net/addgene:35986 ; RRID:Addgene_35986)

  • For your References section:

    In vivo trafficking and targeting of N-cadherin to nascent presynaptic terminals. Jontes JD, Emond MR, Smith SJ. J Neurosci. 2004 Oct 13;24(41):9027-34. 10.1523/JNEUROSCI.5399-04.2004 PubMed 15483121
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