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pcDNA3.1-TAT-1-101-FLAG basic mutant
(Plasmid #35985)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35985 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    transactivating protein
  • Alt name
    tat; p14
  • Insert Size (bp)
    354
  • Mutation
    tat from HIV1, 101 aa; R to A mutations at aa 56, 57, 59-61
  • GenBank ID
    155871
  • Entrez Gene
    tat (a.k.a. HIV1gp5)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 fwd
  • 3′ sequencing primer BGH rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-TAT-1-101-FLAG basic mutant was a gift from Susana Valente (Addgene plasmid # 35985 ; http://n2t.net/addgene:35985 ; RRID:Addgene_35985)

  • For your References section:

    An Analog of the Natural Steroidal Alkaloid Cortistatin A Potently Suppresses Tat-Dependent HIV Transcription. Mousseau G, Clementz MA, Bakeman WN, Nagarsheth N, Cameron M, Shi J, Baran P, Fromentin R, Chomont N, Valente ST. Cell Host Microbe. 2012 Jul 19;12(1):97-108. 10.1016/j.chom.2012.05.016 PubMed 22817991
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