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Addgene

MDH1-PGK-FLAG-TAT-86-E
(Plasmid #35978)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35978 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MDH1-PGK-GFP_2.0
  • Backbone manufacturer
    Addgene Plasmid Repository
  • Backbone size w/o insert (bp) 6963
  • Vector type
    Mammalian Expression, Retroviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    transactivating protein
  • Alt name
    tat; p14
  • Species
    Human Immunodeficiency Virus
  • Insert Size (bp)
    258
  • Mutation
    From the "E" HIV clade
  • GenBank ID
    155871
  • Entrez Gene
    tat (a.k.a. HIV1gp5)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer mPGK fwd
  • 3′ sequencing primer H1 primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MDH1-PGK-FLAG-TAT-86-E was a gift from Susana Valente (Addgene plasmid # 35978 ; http://n2t.net/addgene:35978 ; RRID:Addgene_35978)

  • For your References section:

    Didehydro-cortistatin A inhibits HIV-1 Tat mediated neuroinflammation and prevents potentiation of cocaine reward in Tat transgenic mice. Mediouni S, Jablonski J, Paris JJ, Clementz MA, Thenin-Houssier S, McLaughlin JP, Valente ST. Curr HIV Res. 2015;13(1):64-79. PubMed 25613133
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