Skip to main content
Addgene

pcDNA3.1-hnRNPU-V5
(Plasmid #35974)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    heterogeneous nuclear ribonucleoprotein U
  • Alt name
    HNRPU; SAF-A; U21.1; hnRNP U
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2630
  • GenBank ID
    NM_004501.3
  • Entrez Gene
    HNRNPU (a.k.a. DEE54, EIEE54, GRIP120, HNRNPU-AS1, HNRPU, SAF-A, SAFA, U21.1, hnRNP U, pp120)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site Agel (not destroyed)
  • 5′ sequencing primer T7 fwd
  • 3′ sequencing primer BGH rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-hnRNPU-V5 was a gift from Susana Valente (Addgene plasmid # 35974 ; http://n2t.net/addgene:35974 ; RRID:Addgene_35974)

  • For your References section:

    Didehydro-cortistatin A inhibits HIV-1 Tat mediated neuroinflammation and prevents potentiation of cocaine reward in Tat transgenic mice. Mediouni S, Jablonski J, Paris JJ, Clementz MA, Thenin-Houssier S, McLaughlin JP, Valente ST. Curr HIV Res. 2015;13(1):64-79. PubMed 25613133
Related items:
Commonly requested with: