Skip to main content
Addgene

pMSCV-Flag-Zfp423-deltaSBD
(Plasmid #35971)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMSCV puro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6295
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zinc Finger Protein 423
  • Alt name
    Oaz, Roaz, Ebfaz
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3284
  • Mutation
    lacks zinc fingers 14-19 (aa 640 -838) (delta SMAD binding domain)
  • GenBank ID
    NM_033327
  • Entrez Gene
    Zfp423 (a.k.a. Ebfaz, Roaz, Zfp104, Znf423, ataxia1, mKIAA0760, nur12)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Agel (not destroyed)
  • 5′ sequencing primer MSCV forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-Flag-Zfp423-deltaSBD was a gift from Bruce Spiegelman (Addgene plasmid # 35971 ; http://n2t.net/addgene:35971 ; RRID:Addgene_35971)

  • For your References section:

    Transcriptional control of preadipocyte determination by Zfp423. Gupta RK, Arany Z, Seale P, Mepani RJ, Ye L, Conroe HM, Roby YA, Kulaga H, Reed RR, Spiegelman BM. Nature. 2010 Mar 3. ():. 10.1038/nature08816 PubMed 20200519
Related items:
Commonly requested with: