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pCR-blunt-TOPO-FNDC5
(Plasmid #35970)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35970 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-Blunt II-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3519
  • Vector type
    cloning vector
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fibronectin type III domain containing 5
  • Alt name
    Fndc5, PeP, Pxp, C87088, AI836596, 1500001L03Rik
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    630
  • GenBank ID
    NM_027402
  • Entrez Gene
    Fndc5 (a.k.a. 1500001L03Rik, PeP, Pxp)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Fndc5 gene has a high GC content and therefore may be difficult to re-clone by PCR, restriction digest should be used in re-cloning attempts.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR-blunt-TOPO-FNDC5 was a gift from Bruce Spiegelman (Addgene plasmid # 35970 ; http://n2t.net/addgene:35970 ; RRID:Addgene_35970)

  • For your References section:

    A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, Rasbach KA, Bostrom EA, Choi JH, Long JZ, Kajimura S, Zingaretti MC, Vind BF, Tu H, Cinti S, Hojlund K, Gygi SP, Spiegelman BM. Nature. 2012 Jan 11;481(7382):463-8. doi: 10.1038/nature10777. 10.1038/nature10777 PubMed 22237023
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