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Addgene

pg50-2.11 (#65)
(Plasmid #35966)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK (+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    This plasmid is not very stable (the bacteria lose or even rearrange it quite often). Pool miniprep-DNA from freshly transformed bacteria instead of midi- or maxiprep.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tie2 promoter-1st exon-1st intron
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    17000

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TIE2 genomic subclone DNA containing the 10kb enhancer fragment (NaeI-SalI or NotI)

The depositing laboratory recommends digesting plasmid DNA obtained by minipreps with NaeI+SalI or NotI to verify the presence of the 10 kb enhancer fragment.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pg50-2.11 (#65) was a gift from Thomas Sato (Addgene plasmid # 35966 ; http://n2t.net/addgene:35966 ; RRID:Addgene_35966)

  • For your References section:

    Uniform vascular-endothelial-cell-specific gene expression in both embryonic and adult transgenic mice. Schlaeger TM, Bartunkova S, Lawitts JA, Teichmann G, Risau W, Deutsch U, Sato TN. Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3058-63. 10.1073/pnas.94.7.3058 PubMed 9096345
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