pT2HLacZpA1I.7 (#59)
(Plasmid #35965)

• Depositing Lab: Thomas Sato
• Publication: Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3058-63.

Backbone

• Vector backbone: pBluescript II SK (+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Tie2 promoter
• Species: M. musculus (mouse)
• Entrez Gene: Tek (a.k.a. Cd202b, Hyk, STK1, Tie-2, Tie2)

Gene/Insert 2

• Gene/Insert name: SDK LacZ

Gene/Insert 3

• Gene/Insert name: SV40 pA

Gene/Insert 4

• Gene/Insert name: Tie2 full enhancer
• Species: M. musculus (mouse)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Positive control DNA where LacZ is expressed under the control of Tie2 promoter and enhander using the longer enhancer fragment as indicated in the map. Insert DNA fragments can be excised by SalI digest.