pSPTg.T2FpAXK (#52)
(Plasmid #35962)

• Depositing Lab: Thomas Sato
• Publication: Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3058-63.

Backbone

• Vector backbone: pSP72
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2462

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Tie2 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 2000

Gene/Insert 2

• Gene/Insert name: SV40 pA
• Insert Size (bp): 300

Gene/Insert 3

• Gene/Insert name: Tie2 enhancer
• Species: M. musculus (mouse)
• Insert Size (bp): 1600

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector contains Tie2 promoter, SV40 polyA signal and Tie2 minimum enhancer fragment. MCS (HindIII and NotI) are located between the promoter and pA signal where you can insert your cDNA as indicated. DNA fragments can be excised by SalI digest from injection.