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pAC-4CL1
(Plasmid #35947)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35947 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAC
  • Backbone size w/o insert (bp) 4449
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4-coumaroyl CoA ligase 1
  • Alt name
    4CL
  • Alt name
    4CL1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1722
  • GenBank ID
    U18675.1
  • Entrez Gene
    4CL1 (a.k.a. AT1G51680, 4-COUMARATE:COA LIGASE, 4-COUMARATE:COA LIGASE 1, 4-coumarate:CoA ligase 1, 4CL.1, ARABIDOPSIS THALIANA 4-COUMARATE:COA LIGASE 1, AT4CL1, F19C24.11, F19C24_11)
  • Promoter pLac-mod

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer TGT TGT GTG GAA TTG TGA GCG TCT AGA
  • 3′ sequencing primer GCA CAG ATG CGT AAG GAG AAA ATA CC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Initial gene cloning described in the following:
"Exploring Recombinant Flavonoid Biosynthesis in Metabolically Engineered Escherichia coli" (2004)Kevin T. Watts, Pyung Cheon Lee Dr., Claudia Schmidt-Dannert Prof. ChemBioChem. DOI: 10.1002/cbic.200300783

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-4CL1 was a gift from Claudia Schmidt-dannert (Addgene plasmid # 35947 ; http://n2t.net/addgene:35947 ; RRID:Addgene_35947)

  • For your References section:

    Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli. Watts KT, Lee PC, Schmidt-Dannert C. BMC Biotechnol. 2006 Mar 21;6:22. 10.1186/1472-6750-6-22 PubMed 16551366
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