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Addgene

Wnt3A STOP
(Plasmid #35871)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35871 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR/D-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2580
  • Vector type
    Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Wnt3A
  • Alt name
    Wnt3A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1065
  • Mutation
    None
  • Entrez Gene
    WNT3A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 (-20)-FWD
  • 3′ sequencing primer M13-Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Wnt3A STOP was a gift from Marian Waterman (Addgene plasmid # 35871 ; http://n2t.net/addgene:35871 ; RRID:Addgene_35871)

  • For your References section:

    A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML. Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. 10.1016/j.diff.2012.06.004 PubMed 22784633
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